Diptera belonging to four different families, as well as four associated parasitoids were reared from fruits of Inga vera Willd. (Fabaceae) in Lavras, Minas Gerais, southeast Brazil. Four hundred and fifty fruits were collected from five trees of I. vera between 2020 and 2021 in various localities situated within the University Federal of Lavras. Specimens belonging to four different dipteran families were reared: Anastrepha distincta Greene (Tephritidae), Asteromyia sp. (Cecidomyiidae), Drosophila zottii Vilela (Drosophilidae), and Neosilba pendula (Bezzi) (Lonchaeidae). Lopheucoila sp. (Hymenoptera: Figitidae) probably is a parasitoid of N. pendula whereas Eupelmussp. (Hymenoptera: Eupelmidae), and two species of Sycophila (Hymenoptera, Eurytomidae) appear to be parasitoids of Asteromyiasp. Additionally, the majority of the species reared in this study were characterized by DNA barcoding. This study provides valuable insights into the biology and ecology of frugivorous flies in Brazil, and sheds light on potential biological control agents, enabling better management practices.
