Bertholletia excelsa (Brazil nut) is the only representative of the Bertholletia genus, but presents great phenotypic variability. The objective of this study was to characterize the fruits, seeds and crude oil of Brazil nuts from the state of Mato Grosso, Brazil. Fruit and seeds from adult trees were collected at the municipalities of Alta Floresta, Cotriguaçu, Itaúba and Juína. Physical characteristics, yield and composition of the seeds, as well as crude oil characteristics were evaluated. The fruits produced in Itaúba were the smallest and had the lowest number of seeds, while those from Juína presented larger size and mass. The nuts produced in Itaúba had the lowest mean unit mass and ten-seed mass values. The seeds produced in Juína presented higher lipid content and lower ash content. The selenium content of the nuts was higher in Cotriguaçu. There were no significant differences in fruit peel thickness, yield and protein content of the nuts or characteristics of the crude oil.
