For the formation of eucalyptus are used substrates of differentcompositions, which often have no mycorrhizal inoculum. The presenceof mycorrhizal fungi in the substrate can assist in the development ofseedlings and the establishment and maintenance of these aftertransplantation. The objective of this study was to evaluate the populationand diversity of arbuscular mycorrhizal fungi and ectomycorrhizal seedlingsin the market in the municipality of Santa Maria, RS. The results concludingthat there is occurrence of spores of mycorrhizal fungi on substrates ofeucalyptus seedlings, but the association with the plants was low. Noformation was observed in ectomycorrhizal seedlings of eucalyptus assessed.