This work aimed to evaluate the development and quality of Eucalyptus grandis seedlings produced with different dosages of wastewater from fish farming. The experiment was arranged in a completely randomized design with five treatments and four replicates. The treatments were fish wastewater (FW); daily nutritional solution (DNS), combinations of 50% DNS + 50% FW; 25% DNS + 75% FW and solution with weekly application (WNS). The best growth and development results of the eucalyptus seedlings were observed for the solutions applied daily and weekly. The 50% DNS + 50% FW combination did not differ from daily and weekly nutritional solutions for Dickson’s quality index. Fertigation only with wastewater from fish farming resulted in lower growth and development of the seedlings, but the use of the combination of 50% FW + 50% DNS enabled the production of seedlings of similar quality to those fertigated with nutritional solutions.