Learning about Science and natural phenomena is important from the beginning of Basic Education. With this in mind, this study aims to analyze the implementation of investigative activities to promote scientific initiation in an Early Childhood Education class. This is a qualitative study, of the research-application type, developed from a research project on Science initiation for young children, carried out at a federal public university. It addresses the theme “air and wind,” a suitable topic for working with Inquiry-Based Science Education with children. For this, playful activities were carried out with recyclable materials, taking into account the children’s previous knowledge, and meeting the learning objectives proposed by the Brazilian National Common Curriculum Base for Early Childhood Education. The results indicate that the ways in which the activities were designed and implemented contributed to the development of scientific initiation through investigative activities. The children’s natural curiosity and their ability to reason, investigate, and argue were further stimulated. More studies on Scientific Literacy in Early Childhood Education must be developed to improve teaching methods and techniques that help children appropriate their culture, life context, and understanding of the natural phenomena to which they are exposed.